抗性淀粉检测试剂盒 K-RSTAR Resistant Starch Assay Kit

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抗性淀粉检测试剂盒
英文名：Resistant Starch Assay Kit
货号：K-RSTAR
规格：100 assays per kit
市场价： 3472元

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.
Colourimetric method for the determination of Resistant Starch
in cereal products and feeds
Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)
(2) Aqueous ethanol wash + centrifugation to remove D-glucose +
maltose
(3) Dissolution of resistant starch pellet in KOH and neutralisation
(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose
(glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 120 min (plus overnight incubation)
Detection limit: 2-100% of sample weight
Application examples:
Plant materials, starch samples and other materials
Method recognition:
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Measures enzyme resistant starch
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

FAQ解答

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

 

Amresco 吐温20 Tween-20

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Amresco 吐温20  Tween-20 

          在生命科学领域，上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Amresco 吐温20  Tween-20 的签约代理商，在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Amresco 吐温20  Tween-20 高品质的产品和服务，欢迎广大新老客户来电咨询。

Amresco     Tween-20 吐温-20      规格:100ml   含量98%以上

品牌:Amresco
货号：0777
说明：非离子型表面活性剂。水溶性乳化剂。气相色谱固定液。分离分析挥发油、脂肪酸酯、醇、铜、卤化物。
别名：聚乙氧基月桂酸清凉茶醇；Polyoxyethylene-20- sorbitan monolaurate；T-20。
CAS＃：9005-64-5
外观：黄色油状液体
溶解性：溶于水，稀酸稀碱及多数有机溶剂，不溶于植物油及矿油

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